汤富酬

汤富酬，男，1998年及2003年分别获北京大学生物学专业理学学士、博士学位。2004年-2010年在英国剑桥大学Gurdon研究所做博士后研究工作，现任北京大学生物动态光学成像中心 (BIOPIC中心) 研究员、清华大学-北京大学生命科学联合中心研究员、北京大学未来基因诊断高精尖创新中心研究员。

2016年8月获国家杰出青年科学基金资助，2016年11月获第九届谈家桢生命科学创新奖。2021年9月13日，获第三届“科学探索奖”。^[1]

汤富酬教授，现任北京未来基因诊断高精尖创新中心研究员，北京大学生命科学学院 BIOPIC 中心教授。2010 年回国在北京大学组建自己的实验室，主要从事人类早期胚胎以及生殖系细胞发育的单细胞功能基因组学研究，做出了一系列国际前沿的成果。在国际上率先系统发展了单细胞功能基因组学研究体系，开启了单细胞转录组测序时代，建立了单细胞转录组高通量测序、单细胞 DNA 甲基化组高通量测序、单细胞染色质状态组高通量测序、单细胞多组学平行高通量测序等技术，并利用这一技术体系对人类早期胚胎以及生殖系细胞进行了深入、系统的分析，发现了人类早期胚胎以及生殖系细胞发育过程中基因表达网络的重要表观遗传学调控机理。例如，发现人类植入前胚胎的DNA甲基化重编程过程是基因组全局大规模去甲基化和重要基因组区域从头加甲基化之间复杂动态平衡的结果。还发现了人类胚胎生殖细胞异步发育过程中的全部重要阶段和关键节点以及生殖细胞-微环境细胞之间的协同发育关系，深化了对人类早期胚胎和生殖系细胞发育以及表观遗传重编程过程的认识。发表论文 70 余篇，其中 50 余篇论文是以通讯（或者共同通讯）作者身份发表在Cell（2013；2015），Nature（2014；2016；2018），Science（2015；2018），Cell Stem Cell（2014；2017a；2017b；2017c；2018），Nature Genetics（2018），Nature Cell Biology(2018a；2018b)，Genome Research（2013）等国际学术期刊上。文章已经被引用 8000 多次。其中两项研究工作获评 2014 年度中国科学十大进展和 2015 年度中国科学十大进展。

汤富酬教授 1998 年获得北京大学细胞生物学及遗传学系学士学位，2003 年获得该系博士学位。2010 年在北京大学 BIOPIC 组建的实验室在人类早期胚胎以及生殖系细胞的表观遗传学重编程方面取得了一系列重要研究成果，获得国际同行的广泛认可。担任《科学通报》英文版编委（2014-2017）、Genome Biology编委、Open Biology编委。2013 年获得国家自然科学基金委优秀青年基金、 2016 年获得国家自然科学基金委杰出青年基金。还获得吴杨奖基础医学奖、谈家桢生命科学创新奖、国家科学技术进步奖二等奖、普洛麦格生物化学奖、转化医学创新奖、顾孝诚讲座奖等奖励。多次受邀参加AGBT（Advances in Genome Biology & Technology）、ISSCR（International Society for Stem Cell Research）、ICHG（International Congress of Human Genetics）、Gordon Conference、AACR（American Association for Cancer Research）、HCA（Human Cell Atlas）等国际知名学术大会并作受邀报告。还组织并主持了冷泉港亚洲单细胞基因组学前沿国际会议（2016；2018）。

汤富酬课题组在 ICG 主要从事人类早期胚胎各主要器官以及生殖系细胞的单细胞功能基因组学研究，同时进一步发展和完善单细胞功能基因组学高通量测序技术体系，以深化对人类胚胎发育过程中基因表达网络的遗传学和表观遗传学调控机理的理解，促进解决相关临床疾病的诊断和治疗问题。

1994 - 1998 本科, 遗传学, 北京大学

1998 - 2003 博士, 细胞生物学, 北京大学

2004 - 2010 英国剑桥大学Gurdon研究所，博士后

2010 - 现在　研究员,北京大学生命科学学院生物医学前沿创新中心（BIOPIC）

2015 - 现在　研究员, 北大-清华生命科学联合中心

2017 - 现在　教授, 北京大学生命科学学院生物医学前沿创新中心（BIOPIC）

2018年，荣获第十九届吴阶平-保罗·杨森医学药学奖（吴杨奖）^[2]

2018, "人类胚胎发育机制研究取得新进展"项目成果入选“2017年度中国十大医学科技新闻”

2017, 拜尔学者奖

2016，第九届谈家桢zhēn生命科学创新奖

2016，第二届普洛麦格生物化学奖

2016，国家自然科学基金委，杰出青年基金

2016，“揭示人类原始生殖细胞基因表达与表观遗传调控特征”项目成果入选“2015年度中国科学十大进展”

2015，“顾孝诚讲座奖”

2015，“精确推演母源基因组信息”入选"2014年度中国科学十大进展"

2013，国家自然科学基金委，优秀青年基金

2021年9月13日，获第三届“科学探索奖”（生命科学）^[1]

具有自我更新能力和分化潜能的干细胞是哺乳动物胚胎发育过程中以及成体中的关键种类的细胞，对各种干细胞进行深入研究是理解哺乳动物发育、生长机制的关键，也是将干细胞应用于临床再生医学、治疗人类疾病的前提。

课题围绕人类早期胚胎发育、研究多能干细胞的自我更新能力和多能性调控的分子机理，特别是表观遗传学调控机理，以及相关的原始生殖细胞发育过程中的表观遗传学重编程机理。

利用单细胞功能基因组学分析技术（单细胞 RNA-Seq 转录组分析技术、单细胞 DNA甲基化组测序技术、单细胞多组学平行测序技术等），以及基因编辑技术、少量细胞染色体免疫共沉淀-高通量测序技术、单细胞基因组测序技术、小鼠胚胎显微操作技术和胚胎干细胞体外定向分化等技术在单细胞和单碱基分辨率深入分析人类早期胚胎、生殖系细胞、以及多能性干细胞中基因表达网络的表观遗传学调控机理。

代表性论文

1. Chen Y, Lyu R, Rong B, Zheng Y, Lin Z, Dai R, Zhang X, Xie N, Wang S, Tang Fuchou*, Lan F*, Tong MH*. Refined spatial temporal epigenomic profiling reveals intrinsic connection between PRDM9-mediated H3K4me3 and the fate of double-stranded breaks. Cell Research, doi: 10.1038/s41422-020-0281-1 (2020) (*: Co-corresponding authors).2. Wang S, Zheng Y, Li J, Yu Y, Zhang W, Song M, Liu Z, Min Z, Hu H, Jing Y, He X, Sun L, Ma L, Esteban CR, Chan P, Qiao J, Zhou Q, Izpisua Belmonte JC*, Qu J*, Tang Fuchou*, Liu GH*. Single-Cell Transcriptomic Atlas of Primate Ovarian Aging. Cell, 180: 585-600 (2020) (*: Co-corresponding authors).3. Zhang XM, Wu K, Zheng Y, Zhao H, Gao J, Hou Z, Zhang M, Liao J, Zhang J, Gao Y, Li Y, Li L, Tang Fuchou*, Chen ZJ*, Li J*. In vitro expansion of human sperm through nuclear transfer. Cell Research, doi: 10.1038/s41422-019-0265-1 (2019) (*: Co-corresponding authors).4. Wen L, Liu Q, Xu J, Liu X, Shi C, Yang Z, Zhang Y, Xu H, Liu J*, Yang H*, Huang H*, Qiao J*, Tang Fuchou*, Chen ZJ*. Recent advances in mammalian reproductive biology. Science China Life Sciences, doi: 10.1007/s11427-019-1572-7 (2019) (*: Co-corresponding authors).5. Li J, Wang R, Zhou X, Wang W, Gao S, Mao Y, Wu X, Guo L, Liu H, Wen L, Fu W*, Tang Fuchou*. Genomic and transcriptomic profiling of carcinogenesis in patients with familial adenomatous polyposis. Gut, doi: 10.1136/gutjnl-2019-319438 (2019)(*: Co-corresponding authors).6. Wen L*, Tang Fuchou*. Human Germline Cell Development: from the Perspective of Single-Cell Sequencing. Molecular Cell, 76: 320-328 (2019)(Review)(*: Co-corresponding authors).7. Yang X, Hu B, Liao J, Qiao Y, Chen Y, Qian Y, Feng S, Yu F, Dong J, Hou Y, Xu H, Wang R, Peng G*, Li J*, Tang Fuchou*, Jing N*. Distinct enhancer signatures in the mouse gastrula delineate progressive cell fate continuum during embryo development. Cell Research, 29: 911-926 (2019) (*: Co-corresponding authors).8. Zhou F, Wang R, Yuan P, Ren Y, Mao Y, Li R, Lian Y, Li J, Wen L, Yan L, Qiao J*, Tang Fuchou*. Reconstituting the transcriptome and DNA methylome landscapes of human implantation. Nature, 572: 660-664 (2019) (*: Co-corresponding authors).9. Soares E, Xu Q, Li Q, Qu J, Zheng Y, Raeven HHM, Brandao KO, Petit I, van den Akker WMR, van Heeringen SJ, Aberdam D, Tang Fuchou*, Zhou H*. Single-cell RNA-seq identifies a reversible mesodermal activation in abnormally specified epithelia of p63 EEC syndrome. Proc Natl Acad Sci U S A, 116: 17361-17370 (2019) (*: Co-corresponding authors).10. Hu Y, Wang X, Hu B, Mao Y, Chen Y, Yan L, Yong J, Dong J, Wei Y, Wang W, Wen L, Qiao J*, Tang Fuchou*. Dissecting the transcriptome landscape of the human fetal neural retina and retinal pigment epithelium by single-cell RNA-seq analysis. PLoS Biology, 17: e3000365 (2019) (*: Co-corresponding authors).11. Fu L, Hu Y, Song M, Liu Z, Zhang W, Yu FX, Wu J, Wang S, Izpisua Belmonte JC, Chan P, Qu J*, Tang Fuchou*, Liu GH*. Up-regulation of FOXD1 by YAP alleviates senescence and osteoarthritis. PLoS Biology, 17: e3000201 (2019) (*: Co-corresponding authors).12. Ren X, Hu B, Song M, Ding Z, Dang Y, Liu Z, Zhang W, Ji Q, Ren R, Ding J, Chan P, Jiang C, Ye K, Qu J*, Tang Fuchou*, Liu GH*. Maintenance of Nucleolar Homeostasis by CBX4 Alleviates Senescence and Osteoarthritis. Cell Reports, 26: 3643-3656 (2019) (*: Co-corresponding authors).13. Cui Y, Zheng Y, Liu X, Yan L, Fan X, Yong J, Hu Y, Dong J, Li Q, Wu X, Gao S, Li J, Wen L, Qiao J*, Tang Fuchou*. Single-cell transcriptome analysis maps the developmental track of the human heart. Cell Reports, 26: 1934–1950 (2019) (*: Co-corresponding authors).14. Yan P, Li Q, Wang L, Lu P, Suzuki K, Liu Z, Lei J, Li W, He X, Wang S, Ding J, Chan P, Zhang W, Song M, Izpisua Belmonte JC, Qu J*, Tang Fuchou*, Liu GH*. FOXO3-engineered human ESC-derived vascular cells promote vascular protection and regeneration. Cell Stem Cell, 24: 447-461 (2019) (*: Co-corresponding authors).15. Ji Q, Zheng Y, Zhang G, Hu Y, Fan X, Hou Y, Wen L, Li L, Xu Y, Wang Y*, Tang Fuchou*. Single-cell RNA-seq analysis reveals the progression of human osteoarthritis. Annals of the Rheumatic Diseases, 78: 100-110 (2019) (*: Co-corresponding authors).16. Bian S, Hou Y, Zhou X, Li X, Yong J, Wang Y, Wang W, Yan J, Hu B, Guo H, Wang J, Gao S, Mao Y, Dong J, Zhu P, Xiu D, Yan L, Wen L, Qiao J*, Tang Fuchou*, Fu W*. Single-cell multiomics sequencing and analyses of human colorectal cancer. Science, 362: 1060-1063 (2018) (*: Co-corresponding authors).17. Wang P, Chen Y, Yong J, Cui Y, Wang R, Wen L, Qiao J*, Tang Fuchou*. Dissecting the global dynamic molecular profiles of human fetal kidney development by single-cell RNA sequencing. Cell Reports, 24: 3554-3567 (2018) (*: Co-corresponding authors).18. Wang M, Liu X, Chang G, Chen Y, An G, Yan L, Gao S, Xu Y, Cui Y, Dong J, Chen Y, Fan X, Hu Y, Song K, Zhu X, Gao Y, Yao Z, Bian S, Hou Y, Lu J, Wang R, Fan Y, Lian Y, Tang W, Wang Y, Liu J, Zhao L, Wang L, Liu Z, Yuan R, Shi Y, Hu B, Ren X, Tang Fuchou*, Zhao XY*, Qiao J*. Single-cell RNA sequencing analysis reveals sequential cell fate transition during human spermatogenesis. Cell Stem Cell, 23: 599-614 (2018) (*: Co-corresponding authors).19. Chen Y, Zheng Y, Gao Y, Lin Z, Yang S, Wang T, Wang Q, Xie N, Hua R, Liu M, Sha J, Griswold MD, Li J*, Tang Fuchou*, Tong MH*. Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis. Cell Research, 28: 879–896 (2018) (Cover story) (*: Co-corresponding authors).20. Li L, Guo F, Gao Y, Ren Y, Yuan P, Yan L, Li R, Liang Y, Li J, Hu B, Gao J, Wen L, Tang Fuchou*, Qiao J*. Single-cell multi-omics sequencing of human early embryos. Nature Cell Biology, 20: 847-858 (2018) (*: Co-corresponding authors).21. Fan X, Dong J, Zhong S, Wei Y, Wu Q, Yan L, Yong J, Sun L, Wang X, Zhao Y, Wang W, Yan J, Wang X*, Qiao J*, Tang Fuchou*. Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis. Cell Research, 28: 730-745 (2018) (*: Co-corresponding authors).22. Gao S, Yan L, Wang R, Li J, Yong J, Zhou X, Wei Y, Wu X, Wang X, Fan X, Yan J, Zhi X, Gao Y, Guo H, Jin X, Wang W, Mao Y, Wang F, Wen L, Fu W, Ge H*, Qiao J*, Tang Fuchou*. Tracing the temporal-spatial transcriptome landscapes of the human fetal digestive tract using single-cell RNA-sequencing. Nature Cell Biology, 20: 721-734 (2018) (*: Co-corresponding authors).23. Zhong S, Zhang S, Fan X, Wu Q, Yan L, Dong J, Zhang H, Li L, Sun L, Pan N, Xu X, Tang Fuchou*, Zhang J*, Qiao J*, Wang X*. A single-cell RNA-seq survey of the developmental landscape of the human prefrontal cortex. Nature, 555: 524-528 (2018) (*: Co-corresponding authors).24. Zhu P, Guo H, Ren Y, Hou Y, Dong J, Li R, Lian Y, Fan X, Hu B, Gao Y, Wang X, Wei Y, Liu P, Yan J, Ren X, Yuan P, Yuan Y, Yan Z, Wen L, Yan L*, Qiao J*, Tang Fuchou*. Single-cell DNA methylome sequencing of human preimplantation embryos. Nature Genetics, 50: 12-19 (2018) (*: Co-corresponding authors).25. Wen L*, Tang Fuchou*. Boosting the power of single-cell analysis. Nature Biotechnology, 36: 408-409 (2018) (Preview) (*: Co-corresponding authors).26. Dong J, Hu Y, Fan X, Wu X, Mao Y, Hu B, Guo H, Wen L, Tang Fuchou*. Single-cell RNA-seq analysis unveils a prevalent epithelial/mesenchymal hybrid state during mouse organogenesis. Genome Biology, 19: 31 (2018) (*: Corresponding author).27. Yang X, Hu B, Hou Y, Qiao Y*, Wang R, Chen Y, Qian Y, Feng S, Chen J, Liu C, Peng G, Tang Fuchou*, Jing N*. Silencing of developmental genes by H3K27me3 and DNA methylation reflects the discrepant plasticity of embryonic and extraembryonic lineages. Cell Research, 28: 593-596 (2018) (*: Co-corresponding authors).28. Wang S, Hu B, Ding Z, Dang Y, Wu J, Li D, Liu X, Xiao B, Zhang W, Ren R, Lei J, Hu H, Chen C, Chan P, Li D, Qu J*, Tang Fuchou*, Liu GH*. ATF6 safeguards organelle homeostasis and cellular aging in human mesenchymal stem cells. Cell Discovery, 4: 2 (2018) (*: Co-corresponding authors).29. Wen L* & Tang Fuchou*. Single cell epigenome sequencing technologies. Molecular Aspects of Medicine, 59: 62-69 (2018) (Review) (*: Co-corresponding authors).30. Liu J, Liu W, Yang L, Wu Q, Zhang H, Fang A, Li L, Xu X, Sun L, Zhang J*, Tang Fuchou*, Wang X*. The primate-specific gene TMEM14B marks outer radial glia cells and promotes cortical expansion and folding. Cell Stem Cell, 21: 635-649 (2017) (*: Co-corresponding authors).31. Guo F*, Li L, Li J, Wu X, Hu B, Zhu P, Wen L, Tang Fuchou*. Single-cell multi-omics sequencing of mouse early embryos and embryonic stem cells. Cell Research, 27: 967-988 (2017) (Cover story) (*: Co-corresponding authors).32. Yang J, Li J, Suzuki K, Liu X, Wu J, Zhang W, Ren R, Zhang W, Chan P, Izpisua Belmonte JC, Qu J*, Tang Fuchou*, Liu GH*. Genetic enhancement in cultured human adult stem cells conferred by a single nucleotide recoding. Cell Research, 27: 1178-1181 (2017) (*: Co-corresponding authors).33. Li L, Dong J, Yan L, Yong J, Liu X, Hu Y, Fan X, Wu X, Guo H, Wang X, Zhu X, Li R, Yan J, Wei Y, Zhao Y, Wang W, Ren Y, Yuan P, Yan Z, Hu B, Guo F, Wen L, Tang Fuchou*, Qiao J*. Single-cell RNA-seq analysis maps development of human germline cells and gonadal niche interactions. Cell Stem Cell, 20: 858-873 (2017) (*: Co-corresponding authors).34. Zhu C, Gao Y, Guo H, Xia B, Song J, Wu X, Zeng H, Kee K, Tang Fuchou*, Yi C*. Single-cell 5-formylcytosine landscapes of mammalian early embryos and ESCs at single-base resolution. Cell Stem Cell, 20: 720-731 (2017) (*: Co-corresponding authors).35. Guo H, Hu B, Yan L, Yong J, Wu Y, Gao Y, Guo F, Hou Y, Fan X, Dong J, Wang X, Zhu X, Yan J, Wei Y, Jin H, Zhang W, Wen L, Tang Fuchou*, Qiao J*. DNA methylation and chromatin accessibility profiling of mouse and human fetal germ cells. Cell Research, 27: 165-183 (2017) (*: Co-corresponding authors).36. Yang L, Ma Z, Cao C, Zhang Y, Wu X, Lee R, Hu B, Wen L, Ge H, Huang Y*, Lao K*, Tang Fuchou*. MR-seq: measuring a single cell’s transcriptome repeatedly by RNA-seq. Science Bulletin, 62: 391-398 (2017) (*: Co-corresponding authors).37. Zhou F, Li X, Wang W, Zhu P, Zhou J, He W, Ding M, Xiong F, Zheng X, Li Z, Ni Y, Mu X, Wen L, Cheng T, Lan Y, Yuan W*, Tang Fuchou*, Liu B*. Tracing haematopoietic stem cell formation at single-cell resolution. Nature, 533: 487-492 (2016) (*: Co-corresponding authors).38. Wen L*, Tang Fuchou*. Single-cell sequencing in stem cell biology. Genome Biology 17: 71 (2016) (*: Co-corresponding authors) (Review).39. Dang Y, Yan L, Hu B, Fan X, Ren Y, Li R, Lian Y, Yan J, Li Q, Zhang Y, Li M, Ren X, Huang J, Wu Y, Liu P, Wen L, Zhang C, Huang Y*, Tang Fuchou*, Qiao J*. Tracing the expression of circular RNAs in human pre-implantation embryos. Genome Biology 17: 130 (2016) (*: Co-corresponding authors).40. Dai HQ, Wang BA, Yang L, Chen JJ, Zhu GC, Sun ML, Ge H, Wang R, Chapman DL, Tang Fuchou, Sun X, Xu GL. TET-mediated DNA demethylation controls gastrulation by regulating Lefty-Nodal signalling. Nature 538: 528-532 (2016).41. Hou Y, Guo H, Cao C, Li X, Hu B, Zhu P, Wu X, Wen L, Tang Fuchou*, Huang Y*, Peng J*. Single-cell triple omics sequencing reveals genetic, epigenetic, and transcriptomic heterogeneity in hepatocellular carcinomas. Cell Research 26: 304-319 (2016) (*: Co-corresponding authors).42. Pan H, Guan D, Liu X, Li J, Wang L, Wu J, Zhou J, Zhang W, Ren R, Zhang W, Li Y, Yang J, Hao Y, Yuan T, Yuan G, Wang H, Ju Z, Mao Z, Li J, Qu J*, Tang Fuchou*, Liu GH*. SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2. Cell Research 26: 190-205 (2016) (*: Co-corresponding authors).43. Yan L, Guo H, Hu B, Li R, Yong J, Zhao Y, Zhi X, Fan X, Guo F, Wang X, Wang W, Wei Y, Wang Y, Wen L, Qiao J*, Tang Fuchou*. Epigenomic landscape of human fetal brain, heart, and liver. Journal Biological Chemistry 291: 4386-4398 (2016) (*: Co-corresponding authors).44. Yan L, Huang L, Xu L, Huang J, Ma F, Zhu X, Tang Y, Liu M, Lian Y, Liu P, Li R, Lu S, Tang Fuchou*, Qiao J*, Xie XS*. Live births after simultaneous avoidance of monogenic diseases and chromosome abnormality by next-generation sequencing with linkage analyses. Proc Natl Acad Sci U S A 112: 15964-15969 (2015) (*: Co-corresponding authors).45. Wen L*, Tang Fuchou*, How to catch rare cell types. Nature 52: 197-198 (2015) (*: Co-corresponding authors) (Preview).46. Guo F, Yan L, Guo H, Li L, Hu B, Zhao Y, Yong J, Hu Y, Wang X, Wei Y, Wang W, Li R, Yan J, Zhi X, Zhang Y, Jin H, Zhang W, Hou Y, Zhu P, Li J, Zhang L, Liu S, Ren Y, Zhu X, Wen L, Gao Y, Tang Fuchou*, Qiao J*. The transcriptome and DNA methylome landscapes of human primordial germ cells. Cell 161: 1437-1452 (2015) (*: Co-corresponding authors).47. Zhang W, Li J, Suzuki K, Qu J, Wang P, Zhou J, Liu X, Ren R, Xu X, Ocampo A, Yuan T, Yang J, Li Y, Shi L, Guan D, Pan H, Suan S, Ding Z, Li M, Yi F, Bai R, Wang Y, Chen C, Yang F, Li X, Wang Z, Aizawa E, Goebl A, Soligalla RE, Reddy P, Esteban CR, Tang Fuchou*, Liu G* and Izpisua Belmonte JC*. A Werner syndrome stem cell model unveils heterochromatin alterations as a driver of human aging. Science 348: 1160-1163 (2015) (*: Co-corresponding authors).48. Wen L*, Tang Fuchou*. Charting a map through the cellular reprogramming landscape. Cell Stem Cell 16: 215-216 (2015) (*: Co-corresponding authors) (Preview).49. Wen L, Li J, Guo H, Liu X, Zheng S, Zhang D, Zhu W, Qu J, Guo L, Du D, Jin X, Zhang Y, Gao Y, Shen J, Ge H, Tang Fuchou*, Huang Y*, Peng J*. Genome-scale detection of hypermethylated CpG islands in circulating cell-free DNA of hepatocellular carcinoma patients. Cell Research 25: 1250-1264 (2015) (*: Co-corresponding authors).50. Duan S, Yuan G, Liu X, Ren R, Li J, Zhang W, Wu J, Xu X, Fu L, Li Y, Yang J, Zhang W, Bai R, Yi F, Suzuki K, Gao H, Esteban CR, Zhang C, Izpisua Belmonte JC, Chen Z, Wang X, Jiang T, Qu J*, Tang Fuchou*, Liu GH*. PTEN deficiency reprogrammes human neural stem cells towards a glioblastoma stem cell-like phenotype. Nature Communications 6: 10068 (2015) (*: Co-corresponding authors).51. Fan X, Zhang X, Wu X, Guo H, Hu Y, Tang Fuchou*, Huang Y*. Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos. Genome Biology 16: 148 (2015) (*: Co-corresponding authors).52. Guo H, Zhu P, Guo F, Li X, Wu X, Fan X, Wen L*, Tang Fuchou*. Profiling DNA methylome landscapes of mammalian cells with single-cell reduced-representation bisulfite sequencing. Nature Protocols 10: 645-659 (2015) (*: Co-corresponding authors).53. Wu Y, Zhou H, Fan X, Zhang Y, Zhang M, Wang Y, Xie Z, Bai M, Yin Q, Liang D, Tang W, Liao J, Zhou C, Liu W, Zhu P, Guo H, Pan H, Wu C, Shi H, Wu L* , Tang Fuchou*, Li J,* Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells. Cell Research 25: 67-79 (2015) (*: Co-corresponding authors).54. Shen J, Jiang D, Fu Y, Wu X, Guo H, Feng B, Pang Y, Streets AM, Tang Fuchou*, Huang Y*. H3K4me3 epigenomic landscape derived from ChIP-Seq of 1 000 mouse early embryonic cells. Cell Research 25: 143-147 (2015) (*: Co-corresponding authors).55. Guo F, Li X, Liang D, Li T, Zhu P, Guo H, Wu X, Wen L, Gu TP, Hu B, Walsh CP, Li J*, Tang Fuchou*, Xu GL*. Active and passive demethylation of male and female pronuclear dna in the mammalian zygote. Cell Stem Cell 15: 447-458 (2014) (*: Co-corresponding authors).56. Guo H, Zhu P, Yan L, Li R, Hu B, Lian Y, Yan J, Ren X, Lin S, Li J, Jin X, Shi X, Liu P, Wang X, Wang W, Wei Y, Li X, Guo F, Wu X, Fan X, Yong J, Wen L, Xie SX, Tang Fuchou*, Qiao J*. The DNA methylation landscape of human early embryos. Nature 511: 606-610 (2014) (*: Co-corresponding authors).57. Wen L, Tang Fuchou*. Reconstructing complex tissues from single-cell analyses. Cell 157: 771-773 (2014) (*: Corresponding author) (Preview).58. Streets AM, Zhang X, Cao C, Pang Y, Wu X, Xiong L, Yang L, Fu Y, Zhao L*, Tang Fuchou*, Huang Y*. Microfluidic single-cell whole-transcriptome sequencing. Proc Natl Acad Sci U S A 111: 7048-7053 (2014) (*: Co-corresponding authors).59. Wen L, Li X, Yan L, Tan Y, Li R, Zhao Y, Wang Y, Xie J, Zhang Y, Song C, Yu M, Liu X, Zhu P, Li X, Hou Y, Guo H, Wu X, He C*, Li R*, Tang Fuchou*, Qiao J*. Whole-genome analysis of 5-hydroxymethylcytosine and 5-methylcytosine at base resolution in the human brain. Genome Biology 15: R49 (2014) (*: Co-corresponding authors).60. Hou Y, Fan W, Yan L, Li R, Lian Y, Huang J, Li J, Xu L, Tang Fuchou*, Xie XS*, Qiao J*. Genome analyses of single human oocytes. Cell 155:1492-1506 (2013) (*: Co-corresponding authors).61. Guo H, Zhu P, Wu X, Li X, Wen L, Tang Fuchou*. Single-cell methylome landscapes of mouse embryonic stem cells and early embryos analyzed using reduced representation bisulfite sequencing. Genome Research 23: 2126-2135 (2013) (*: Corresponding author).62. Yan L, Yang M, Guo H, Yang L, Wu J, Li R, Liu P, Lian Y, Zheng X, Yan J, Huang J, Li M, Wu X, Wen L, Lao K, Li R*, Qiao J*, Tang Fuchou*. Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells. Nature Structural & Molecular Biology 20: 1131-1139 (2013) (*: Co-corresponding authors).63. Gan H, Wen L, Liao S, Lin X, Ma T, Liu J, Song CX, Wang M, He C, Han C*, Tang Fuchou*. Dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis. Nature Communications 4: 1995 (2013) (*: Co-corresponding authors).64. Tang Fuchou*, Barbacioru C*, Nordman E, Bao S, Lee C, Wang X, Tuch BB, Heard E, Lao K, Surani MA. Deterministic and stochastic allele specific gene expression in single mouse blastomeres. PLoS One 6: e21208 (2011) (*: Co-first authors).65. Tang Fuchou, Lao K, Surani MA. Development and applications of single-cell transcriptome analysis. Nature Methods 8: S6-S11 (2011) (Review).66. Tang Fuchou, Barbacioru C, Bao S, Lee C, Nordman E, Wang X, Lao K, Surani MA. Tracing the derivation of embryonic stem cells from the inner cell mass by single-cell RNA-Seq analysis. Cell Stem Cell 6: 468 - 478 (2010).67. Tang Fuchou*. Small RNAs in mammalian germline: tiny for immortal. Differentiation 79: 141-146 (2010) (*: Corresponding author) (Review).68. Tang Fuchou, Barbacioru C, Nordman E, Li B, Xu N, Bashkirov VI, Lao K, Surani MA. RNA-Seq analysis to capture the transcriptome landscape of a single cell. Nature Protocols 5: 516-535 (2010).69. Bao S*, Tang Fuchou*, Li X, Hayashi K, Gillich A, Lao K, Surani MA. Epigenetic reversion of postimplantation epiblast to pluripotent embryonic stem cells. Nature 461: 1292 - 1295 (2009) (*: Co-first authors).70. Tang Fuchou*, Barbacioru C*, Wang Y, Nordman E, Lee C, Xu N, Wang X, Bodeau J, Tuch BB, Siddiqui A, Lao K, Surani MA. mRNA-Seq whole transcriptome analysis of a single cell. Nature Methods 6: 377 - 382 (2009) (*: Co-first authors).71. Tang Fuchou, Hajkova P, O`Carroll D, Lee C, Tarakhovsky A, Lao K, Surani MA. MicroRNAs are tightly associated with RNA-induced gene silencing complexes in vivo. Biochemical & Biophysical Research Communications 372: 24 - 29 (2008).72. Tang Fuchou, Hayashi K, Kaneda M, Lao K, Surani MA. A sensitive multiplex assay for piRNA expression. Biochemical & Biophysical Research Communications 369: 1190 - 1194 (2008).73. Tang Fuchou*, Kaneda M*, O`Carroll D, Hajkova P, Barton SC, Sun YA, Lee C, Tarakhovsky A, Lao K, Surani MA. Maternal microRNAs are essential for mouse zygotic development. Genes & Development 21: 644 - 648 (2007) (*: Co-first authors).74. Tang Fuchou, Hajkova P, Barton SC, O`Carroll D, Lee C, Lao K, Surani MA. 220-plex microRNA expression profile of a single cell. Nature Protocols 1: 1154 - 1159 (2006).75. Tang Fuchou, Hajkova P, Barton SC, Lao K, Surani MA. MicroRNA expression profiling of single whole embryonic stem cells. Nucleic Acids Research 34: e9 (2006).