汪凯

汪凯，男，理学博士，毕业于中国科学院上海巴斯德研究所，现就职于重庆医科大学感染性疾病分子生物学教育部重点实验室，任副研究员，博士生导师。^[1][2]

2002.9-2006.7 新疆大学 生命科学与技术学院 生物科学 理学学士

2006.9-2012.1 中国科学院 上海巴斯德研究所 微生物学 理学博士

2013.7-2015.7 中国科学院 上海生命科学研究院 生物学 博士后^[3]

2012.5-2013.5 中科院上海巴斯德研究所 分子病毒与免疫重点实验室 助理研究员^[4]

2015.9-2019.9 重庆医科大学 基础医学院病原生物学教研室 讲师

2019.10-2020.10 重庆医科大学 感染性疾病分子生物学重点实验室 副教授

2020.10- 重庆医科大学 感染性疾病分子生物学重点实验室 副研究员^[5]

(1)分子病毒学——感染与代谢重塑；

(2)代谢重编程调控肝癌发生发展的分子机制研究。^[2]

中国抗癌协会肿瘤代谢专委会青年委员；

重庆抗癌协会化疗专业委员会委员；

Advanced Science、Nat Commun、Clin Transl Med、Genes & Disease等学术期刊审稿人学术期刊审稿人。^[2]

汪凯主要从事分子病毒学及肝癌代谢研究；围绕代谢与信号转导的交互调控，旨在揭示代谢重编程在肝癌发生发展中的作用，为肝癌治疗提供新的靶点^[6]。重医未来医学青年创新团队“病毒感染与代谢重塑研究团队”负责人，先后主持国家自然科学基金3项；重庆市科委及教委课题2项，重庆医科大学“新冠肺炎”应急课题等；获得国家发明专利1项^[2]。

科研项目

1.国家自然科学基金 (面上项目)，糖异生代谢酶PCK1上调组蛋白H3K9me3修饰抑制肝癌的机制研究，主持（在研）。

2.国家自然科学基金 (面上项目)，PCK1通过降低O-GlcNAc糖基化修饰抑制肝癌转移的机制研究，主持（在研）^[7]。

3.国家自然科学基金 (青年基金)，PCK1通过Wnt信号通路抑制肝细胞癌的作用机制研究，主持 （已结题）^[8]。

4.重庆医科大学 未来医学青年创新团队发展支持计划，“病毒感染与代谢重塑研究团队”，主持（在研）。

5.重庆医科大学“新型冠状病毒肺炎”应急专项，SARS-CoV-2假病毒系统的建立及其在抗病毒药物筛选中的应用，主持（已结题）。

6.重庆市教委科学技术研究计划，PCK1下调β-catenin稳定性抑制肝癌EMT的分子机制研究，主持（已结题）。

7.重庆市教育委员会雏鹰计划研究项目，糖异生关键酶抑制肝癌细胞增殖和迁移能力初探，主持（已结题）。^[2]

2021年 重庆医科大学“优秀科技成果奖”

2021年 爱思唯尔Elsevier“中国金色开放获取高下载论文学者”

2021年 第二十一届重庆市期刊优秀论文 年度优秀论文 一等奖

2023年 MCE中国生命科学研究促进奖-“春华”季 优质科研奖

2023年 川渝科技学术大会优秀论文 特等奖 (排名第三)

在国内外期刊发表学术论文50余篇，其中以第一作者或通讯作者（含共同）在Journal of Clinical Investigation、EMBO Journal、Cell Research、Cell Death & Differentiation、Clinical Infectious Diseases、Signal Transduct Target Ther.和Oncogene等期刊发表SCI论文20余篇（其中2篇为ESI高被引论文），总引用1700余次，谷歌学术H-指数为21。^[2]

代表性论文：

Jin Xiang#, Chang Chen#, Rui Liu#, Dongmei Gou#, Lei Chang, Haijun Deng, Qingzhu Gao, Wanjun Zhang, Lin Tuo, Xuanming Pan, Li Liang, Jie Xia, Luyi Huang, Ke Yao, Bohong Wang, Zeping Hu, Ailong Huang*, Kai Wang*, Ni Tang*, Gluconeogenic enzyme PCK1 deficiency promotes CHK2 O-GlcNAcylation and hepatocellular carcinoma growth upon glucose deprivation. Journal of Clinical Investigation, 2021. (*Co-corresponding author, IF=19.4)Rui Liu#, Dongmei Gou#, Jin Xiang#, Xuanming Pan, Qingzhu Gao, Peng Zhou, Yi Liu, Jie Hu, Kai Wang*, Ni Tang*, O-GlcNAc modified-TIP60/KAT5 is required for PCK1 deficiency-induced HCC metastasis. Oncogene, 2021. (*Co-corresponding author, IF=8.8)Qingzhu Gao#, Bin Cheng#, Chang Chen#, Chong Lei#, Xue Lin, Dan Nie, Jingjing Li, Luyi Huang, Xiaosong Li, Kai Wang*, Ailong Huang*, Ni Tang*, Dysregulated glucuronic acid metabolism exacerbates hepatocellular carcinoma progression and metastasis through the TGFβ signaling pathway. Clinical and Translational Medicine, 2022.  (*Co-corresponding author, IF=8.5)Binli Mao#, Vu Thuy Khanh Le-Trilling#, Kai Wang#, Denise Mennerich, Jie Hu, Zhenyu Zhao, Jiaxin Zheng, Yingying Deng, Benjamin Katschinski, Shilei Xu, Guiji Zhang, Xuefei Cai, Yuan Hu, Jianwei Wang, Mengji Lu, Ailong Huang*, Ni Tang*, Mirko Trilling*, Yong Lin*，Obatoclax inhibits SARS-CoV-2 entry by altered endosomal acidification and impaired cathepsin and furin activity in vitro. Emerging Microbes & Infections, 2022 (Co-first author, IF=19.5)Jie Hu#, Xiao-yu Wei#, Jin Xiang#, Pai Peng, Feng-li Xu, Kang Wu, Fei-yang Luo, Ai-shun Jin, Liang Fang, Bei-zhong Liu*, Kai Wang*, Ni Tang*, Ai-Long Huang*, Reduced neutralization of SARS-CoV-2 B.1.617 variant by convalescent and vaccinated sera. Genes & Diseases, 2022.  (*Co-corresponding author，IF=7.2)Pai Peng#, Hai-jun Deng#, Jie Hu#, Xiao-yu Wei, Jian-jiang Xue, Ting-ting Li, Liang Fang, Bei-zhong Liu, Ai-shun Jin, Feng-li Xu, Kang Wu, Quan-xin Long, Juan Chen, Kai Wang*, Ni Tang*, Ai-long Huang*, Humoral responses in naive or SARS-CoV-2 experienced individuals vaccinated with an inactivated vaccine. Cell Discovery, 2021. (*Co-corresponding author, IF= 38.0)Qiujie Wang#, Bin Cheng#, Qiang Xue#, Qingzhu Gao, Ailong Huang*, Kai Wang*, Ni Tang*, GSTZ1 sensitizes hepatocellular carcinoma cells to sorafenib-induced ferroptosis via inhibition of NRF2/GPX4 axis. Cell Death & Disease, 2021, 12(5):426. (*Co-corresponding author, IF=9.6)Yi Liu#, Haijun Deng#, Li Liang, Guiji Zhang, Jie Xia, Keyue Ding, Ni Tang*, Kai Wang*, Depletion of VPS35 attenuates metastasis of hepatocellular carcinoma by restraining the Wnt/PCP signaling pathway. Genes & Diseases, 2021, 8(2): 232-240. (*Co-corresponding author，IF=7.2)Chang-Long He#, Lu-Yi Huang#, Kai Wang#, Chen-Jian Gu, Jie Hu, Gui-Ji Zhang, Wei Xu, You-Hua Xie*, Ni Tang*, Ai-Long Huang*, Identification of bis-benzylisoquinoline alkaloids as SARS-CoV-2 entry inhibitors from a library of natural products. Signal Transduction and Targeted Therapy, 2021. (#Co-first author, IF=38.1)Jie Hu# , Pai Peng#, Kai Wang#, Liang Fang, Fei-yang Luo, Ai-shun Jin, Bei-zhong Liu*, Ni Tang*, Ai-long Huang*, Emerging SARS-CoV-2 variants reduce neutralization sensitivity to convalescent sera and monoclonal antibodies. Cellular & Molecular Immunology, 2021. (#Co-first author, IF=22.0)Pai Peng#, Jie Hu#, Hai-jun Deng, Bei-zhong Liu, Liang Fang, Kai Wang*, Ni Tang*, Ai-long Huang*, Changes of humoral immunity response in SARS-CoV-2 convalescent patients over 8 months. Cellular & Molecular Immunology, 2021. (*Co-corresponding author, IF=22.0)Jie Hu#, Qingzhu Gao#, Changlong He, Ailong Huang, Ni Tang*, Kai Wang*, Development of cell-based pseudovirus entry assay to identify potential viral entry inhibitors and neutralizing antibodies against SARS-CoV-2. Genes & Diseases, 2020. (*Corresponding author，IF=7.1)Jie Hu#, Qingzhu Gao#, Yang Yang#, Jie Xia#, Wanjun Zhang, Yao Chen, Zhi Zhou, Lei Chang, Yuan Hu, Hui Zhou, Li Liang, Xiaosong Li, Quanxin Long, Kai Wang*, Ailong Huang*, Ni Tang*, Hexosamine biosynthetic pathway promotes the antiviral activity of SAMHD1 by enhancing O-GlcNAc transferase-mediated protein O-GlcNAcylation. Theranostics, 2021. (*Co-corresponding author, IF=11.5)Qingzhu Gao#, Guiji Zhang#, Yaqiu Zheng#, Yi Yang#, Chang Chen, Jie Xia, Li Liang, Chong Lei, Yuan Hu, Xue-Fei Cai, Wenlu Zhang, Hua Tang, Yaxi Chen, Ailong Huang*, Kai Wang*, Ni Tang*, SLC27A5 deficiency activates NRF2/TXNRD1 pathway by increased lipid peroxidation in HCC. Cell Death & Differentiation, 2020.  (*Co-corresponding author, IF=15.8) Fan Yang#, Jingjing Li#, Haijun Deng#, Yihao Wang, Chong Lei, Qiujie Wang, Jin Xiang, Li Liang, Jie Xia, Xuanming Pan, Xiaosong Li, Quanxin Long, Lei Chang, Ping Xu, Ailong Huang*, Kai Wang*, Ni Tang*, GSTZ1-1 Deficiency Activates NRF2/IGF1R Axis in HCC via Accumulation of Oncometabolite Succinylacetone. The EMBO Journal, 2019. (*Co-corresponding author, IF=11.5)  Guiji Zhang#, Xia Tang#, Li Liang#, Wanfeng Zhang, Dewei Li, Xiaoyuan Li, Dachun Zhao, Yaqiu Zheng, Yanhong Chen, Bingtao Hao, Kai Wang*, Ni Tang*, Keyue Ding*, DNA and RNA sequencing identified a novel oncogene VPS35 in liver hepatocellular carcinoma, Oncogene, 2020. (*Co-corresponding author, IF=9.8)  Jin Xiang#, Yuhong Zhang#, Lin Tuo, Rui Liu, Dongmei Gou, Li Liang, Chang Chen, Jie Xia, Ni Tang, Kai Wang*, Transcriptomic changes associated with PCK1 overexpression in hepatocellular carcinoma cells detected by RNA-Seq. Genes & Diseases, 2020. (Corresponding author，IF=7.1)Chong Lei#, Qiujie Wang, Ni Tang*, Kai Wang*, GSTZ1-1 downregulates Wnt/β-catenin signalling in hepatocellular carcinoma cells. FEBS Open Bio, 2020. (Corresponding author，IF=2.7)Jingjing Li#, Qiujie Wang#, Yi Yang, Chong Lei, Fan Yang, Li Liang, Chang Chen, Jie Xia, Kai Wang*, Ni Tang*, GSTZ1 deficiency promotes hepatocellular carcinoma proliferation via activation of the KEAP1/NRF2 pathway. Journal of Experimental & Clinical Cancer Research, 2019. (*Co-corresponding author, IF=11.1)Xuemei Chen#, Yuan Hu#, Wenlu Zhang, Ke Chen, Jie Hu, Xiaosong Li, Li Liang, Xuefei Cai, Jieli Hu, Kai Wang*, Ailong Huang*, Ni Tang*, Cisplatin induces autophagy to enhance hepatitis B virus replication via activation of ROS/JNK and inhibition of the Akt/mTOR pathway. Free Radical Biology and Medicine, 2019. (*Co-corresponding author, IF=7.376)Lin Tuo#, Jin Xiang#, Xuanming Pan#, Jieli Hu, Hua Tang, Li Liang, Jie Xia, Yuan Hu, Wenlu Zhang, Ailong Huang*, Kai Wang*, Ni Tang*, PCK1 negatively regulates cell cycle progression and hepatoma cell proliferation via the AMPK/p27Kip1 axis. Journal of Experimental & Clinical Cancer Research, 2019.(*Co-corresponding author, IF=11.161)  Lin Tuo#, Jin Xiang#, Xuanming Pan, Qingzhu Gao, Guiji Zhang, Yi Yang, Li Liang, Jie Xia, Kai Wang*, Ni Tang*, PCK1 Downregulation Promotes TXNRD1 Expression and Hepatoma Cell Growth via the Nrf2/Keap1 Pathway. Frontiers in Oncology, 2018. (*Co-corresponding author, IF=6.244)Kai Wang#, Quan-Xin Long#, Hai-Jun Deng#, Jie Hu#, Qing-Zhu Gao, Gui-Ji Zhang, Chang-long He, Lu-Yi Huang, Jie-li Hu, Juan Chen*, Ni Tang*, Ai-Long Huang*, Longitudinal Dynamics of the Neutralizing Antibody Response to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection. Clinical Infectious Diseases, 2021. (First author, IF=20.9)Kai Wang#, Wei Lv#, Jianfei Chen#, Shiqi Xie, Hongyan Shi, Haojen Hsu, Wenjing Yu, Ke Xu, Chao Bian, Wolfgang B Fischer, Wolfgang Schwarz, Li Feng*, Bing Sun*, PEDV ORF3 encodes an ion channel protein and regulates virus production. FEBS Letters, 2012.  (First author)Kai Wang#, Shiqi Xie, Bing Sun*. Viral proteins function as ion channels. Biochimica et Biophysica Acta (BBA)-Biomembranes, 2011.  (First author)Shiqi Xie#, Kai Wang#, Wenjing Yu, Wei Lv, Ke Xu, Jianwei Wang, Bin Ye, Wolfgang Schwarz, Qi Jin*, Bing Sun*, DIDS blocks a chloride-dependent current that is mediated by the 2B protein of enterovirus 71, Cell Research, 2011. (#Co-first author)Ronghua Zhang#, Kai Wang#, Wei Lv#, Wenjing Yu, Shiqi Xie, Ke Xu, Wolfgang Schwarz, Sidong Xiong*, Bing Sun*, The ORF4a protein of human coronavirus 229E functions as a viroporin that regulates viral production. Biochimica et Biophysica Acta (BBA)-Biomembranes, 2014. (#Co-first author)Qingzhu Gao#, Kai Wang#, Ke Chen, Li Liang, Yaqiu Zheng, Yunzhi Zhang, Jin Xiang, Ni Tang *, HBx protein-mediated ATOH1 downregulation suppresses ARID2 expression and promotes hepatocellular carcinoma. Cancer Science, 2017. (#Co-first author)Ronghua Zhang#, Kai Wang, Xianqiang Ping, Wenjing Yu, Zhikang Qian, Sidong Xiong*, Bing Sun*, The ns12. 9 accessory protein of human coronavirus OC43 is a viroporin involved in virion morphogenesis and pathogenesis. Journal of Virology, 2015.Silvia Schwarz#, Kai Wang, Wenjing Yu, Bing Sun, Wolfgang Schwarz*, Emodin inhibits current through SARS-associated coronavirus 3a protein. Antiviral Research, 2011.Silvia Schwarz#, Daniel Sauter, Kai Wang, Ronghua Zhang, Bing Sun, Anastasia Karioti, Anna Rita Bilia, Thomas Efferth, Wolfgang Schwarz*, Kaempferol derivatives as antiviral drugs against the 3a channel protein of coronavirus. Planta medica, 2014.Daniel Sauter#, Silvia Schwarz, Kai Wang, Ronghua Zhang, Bing Sun, Wolfgang Schwarz*, Genistein as antiviral drug against HIV ion channel. Planta medica, 2014.向进#，庹tuǒ琳，高庆祝，杨翼，梁利，夏杰，唐霓，汪凯*. PCK1影响肝癌细胞迁移能力的研究. 重庆医科大学学报, 2018.潘璇明#，张桂冀，陈雪梅，梁利，唐霓，汪凯*. 果糖-1，6-二磷酸酶1抑制肝癌细胞株HepG2的自噬及增殖. 中华肝脏病杂志, 2019.林雪#，潘璇明，彭梓珂，汪凯，唐霓*. 葡萄糖-6-磷酸酶催化亚基通过诱导细胞周期阻滞抑制肝癌细胞增殖. 中华肝脏病杂志, 2022.龚德敖#，唐霓，汪凯*. 斑点型锌指结构蛋白在肿瘤中的作用. 生命的化学，2022.^[2]