张弓

张弓，男，1981年生。现任暨南大学生命科学技术学院研究员，暨南大学翻译组学实验室负责人，博士生导师。国家优秀青年基金获得者，国家863青年科学家，国家万人计划“青年拔尖人才”。广东省科技特派员，国际人类蛋白质组计划中国团队骨干研究成员，中国生化与分子生物学学会蛋白质组学专业委员会(CNHUPO)理事，中国分子系统生物学专业委员会委员。获美国化学学会颁发的会员奖。^[1]

张弓教授，2001年获武汉大学生物学学士学位，2005年获汉堡科技大学生物技术硕士学位，2009年6月获博士学位。世人皆知在德国攻读博士学位少有人能按时毕业，而张弓不仅三年攻下博士学位，其博士阶段科研成果挑战1972年诺贝尔化学奖结论，博士论文与答辩更是获得德国最高成绩（summa cum laude，最高荣誉），2010年获德国Michelson-Preis最佳博士生大奖，成为第一位获此荣誉的华人。^[2]

张弓教授研发FANSe系列超高精度大规模测序序列比对算法，是目前精度最高的算法，有效解决大规模测序的可验证性、可重复性问题，并于2018年创造了单机“一小时全基因组、一分钟全外显子组、一秒钟全转录组”分析的世界纪录。作为创始人之一创立深圳承启生物科技有限公司，基于FANSe系列算法建立了世界上第一个高度实用化的基因测序分析云平台，进而打破美国的垄断，建立了第一个全国产化的测序分析全流程，使中国精准医学不再受制于人。在多个紧急临床案例中起到了关键作用，为大众提供低成本、高精度的健康及临床测序信息服务，被邀请作TED演讲。参与中国缅甸远征军遗骸DNA鉴定工作，测定了远征军遗骸的全基因组序列，这是世界上首次成功对超过70年热带地区遗骸进行DNA鉴定，被凤凰卫视、湖南卫视、广东电视台、广州日报等媒体广泛专访报道。

发现翻译速率调控是决定蛋白质折叠的关键因素之一，对1972年诺贝尔化学奖理论“安芬森原则”作出颠覆性更新。首次实现高等生物翻译中mRNA全长测序，揭示了翻译调控在生物信息传递中的关键地位，建立了翻译中mRNA与蛋白质丰度之间的定量关系，将中心法则定量化。由此建立翻译组学新学科领域，率先开发出翻译过程中几乎所有部分的组学测定方法。翻译组分析被作为国际人类蛋白质组计划(HPP)的核心支柱之一。2016年翻译组学被写进国家统编教材，成为生化与分子生物学方向上第一个被写进教科书的由中国人建立的学科领域。在应用方面，发明翻译暂停理性重设计法优化蛋白质可溶性表达效率，可在氨基酸序列、表达条件完全不变的前提下，将蛋白质可溶性表达产率提高数千倍，为生化工程提供全新的可能性。    ^[1]

1Zhang, G., & Ignatova, Z.*, A generic algorithm to predict the speed of translational elongation: implications for protein biogenesis. PLoS One, 4(4):e5036 (2009).2Zhang, G., & Ignatova, Z.*, Isolation of homogenous ribosome-nascent-chain complexes from coupled cell-free transcription-translation system. Nature Protocols doi:10.1038/nprot.2009.47 (2009).3Zhang, G., Hubalewska, M., & Ignatova, Z.*, Transient ribosomal attenuation coordinates protein synthesis and co-translational folding. Nat Struct Mol Biol, 16(3):274-80 (2009).4Czech, A., Fedyunin, I., Zhang, G. & Ignatova, Z.*, Silent mutations in sight: Co-variations in tRNA abundance as key to unravel consequences of silent mutations. Molecular Biosystems. 2010 Oct 1;6(10):1767-725Zhang, G., Fedyunin, I., Miekley, O., Valleriani, A., Moura A. & Ignatova, Z.*, Global and local depletion of ternary complex limits translational elongation. Nucleic Acid Res, (2010) 38(14):4778-876Valleriani, A.*, Zhang, G., Nagar, A., Ignatova, Z., Length dependent translation of messenger RNA by ribosomes, Physical Review E, 83(4 Pt 1):042903 (2011)7Borwankar, T., Rötlein, C., Zhang, G., Techen, A., Dosche, C. & Ignatova, Z.*, Natural osmolytes remodel the aggregation pathway of mutant Huntingtin exon 1. Biochem. 29;50(12):2048-60 (2011).8Zhang, G., Lukoszek, R., Mueller-Roeber, B., Ignatova, Z.*, Different sequence signatures in the upstream regions of plant and animal tRNA genes shape distinct modes of regulation. Nucleic Acid Res. (2011) 39(8):3331-99Zhang, G. & Ignatova, Z.*, Folding at the birth of the nascent chain: Coordinating translation with co-translational folding. Current Opinion of Structural Biology, (2011) 21:25-31.10CL Xiao, XZ Chen, YL Du, ZF Li, L Wei, G Zhang *, QY He *, Dispec: A Novel Peptide Scoring Algorithm Based on Peptide Matching Discriminability, PLoS One (2013) 8 (5), e6272411Wang T *, Cui Y, Jin J, Guo J, Wang G, Yin X, He QY *, Zhang G * , Translating mRNAs strongly correlate to proteins in a multivariate manner and their translation ratios are phenotype specific. Nucleic Acids Res. (2013) 41(9):4743-5412Xiao CL, Chen XZ, Du YL, Sun X, Zhang G*, He QY.* Binomial Probability Distribution Model-Based Protein Identification Algorithm for Tandem Mass Spectrometry Utilizing Peak Intensity Information. J. Proteome Res. (2013) 12 (1), 328–33513Fedyunin I, Lehnhardt L, Böhmer N, Kaufmann P, Zhang G, Ignatova Z*, tRNA concentration fine tunes protein solubility. FEBS Lett. (2012)14Zhang, G.*, Fedyunin, I., Kirchner, S., Xiao, C., Valleriani, A. & Ignatova, Z.*, FANSe: an accurate algorithm for quantitative mapping of large scale sequencing reads. Nucleic Acid Res. (2012) 40(11):e83.15Hinz J., Lehnhardt L., Zakrzewski S., Zhang G., Ignatova Z.*, Polyglutamine expansion alters the dynamics and molecular architecture of aggregates in dentatorubropallidoluysian atrophy, J Biol Chem. (2012) 13;287(3):2068-78.16Chen W, Jin J, Gu W, Wei B, Lei Y, Xiong S *, Zhang G *. Rational design of translational pausing without altering the amino acid sequence dramatically promotes soluble protein expression: A strategic demonstration. Journal of Biotechnology (2014), 189(10), 104-11317Puri P, Wetzel C, Saffert P, Gaston KW, Russell SP, Varela JA, van der Vlies P, Zhang G, Limbach PA, Ignatova Z, Poolman B *, Systematic identification of tRNAome and its dynamics in Lactococcus lactis. Molecular Microbiology (2014), 93(5), 944-95618Chen M, Liu T, Xu L, Gao X, Liu X, Wang C, He Q, Zhang G, Liu L *, Direct Interaction of 14-3-3ζ with Ezrin Promotes Cell Migration by Regulating the Formation of Membrane Ruffle. Journal of Molecular Biology (2014), 426(18), 3118-313319X Wu, L Xu, W Gu, Q Xu, QY He *, X Sun *, G Zhang * Iterative Genome Correction Largely Improves Proteomic Analysis of Nonmodel Organisms. Journal of proteome research (2014), 13 (6), 2724–273420CL Xiao, ZB Mai, XL Lian, JY Zhong, JJ Jin, QY He *, G Zhang *, FANSe2: A Robust and Cost-Efficient Alignment Tool for Quantitative Next-Generation Sequencing Applications, PLoS One (2014), 9(4):e9425021L Zhang, HL Jia, et al., Monitoring of the serum proteome in Kawasaki disease patients before and after immunoglobulin therapy, Biochemical and Biophysical Research Communications (2014), 447(1), 19-2522G Zhang *, T Wang *, QY He *, How to discover new proteins—translatome profiling, （中文版: 怎样发现未知蛋白质——翻译组测序） Science China Life Sciences (2014) 57 (3), 358-36023C Chang, L Li, et al., Systematic analyses of the transcriptome, translatome, and proteome provide a global view and potential strategy for the C-HPP, Journal of proteome research (2014) 13 (1), 38-4924Q Wang, B Wen, et al., Omics Evidence: Single Nucleotide Variants Transmissions on Chromosome 20 in Liver Cancer Cell Lines, Journal of proteome research (2014) 13 (1), 200-21125Y Liu, W Ying, et al, Chromosome-8-Coded Proteome of Chinese Chromosome Proteome Data Set (CCPD) 2.0 with Partial Immunohistochemical Verifications, Journal of proteome research (2014) 13 (1), 126-13626C Zhang, N Li, et al., Systematic analysis of missing proteins provides clues to help define all of the protein-coding genes on human chromosome 1, Journal of proteome research (2014) 13 (1), 114-12527J Zhong, Y Cui, J Guo, Z Chen, L Yang, QY He, G Zhang *, T Wang *, Resolving Chromosome-Centric Human Proteome with Translating mRNA Analysis: A Strategic Demonstration, Journal of proteome research (2014) 13 (1), 50-5928Fan X, Xiong H, Wei J, Gao X, Feng Y, Liu X, Zhang G, He QY * Xu J *, Liu L *. Cytoplasmic hnRNPK interacts with GSK3β and is essential for the osteoclast differentiation . Scientific Reports (2015) 5:17732.29Luo B, Gu W, Zhong J, Wang Y *, Zhang G *. Revealing crosstalk of plant and fungi in the symbiotic roots of sewage-cleaning Eichhornia crassipes using direct de novo metatranscriptomic analysis. Scientific Reports (2015) 5:15407.30Yang L, Lian X, Zhang W, Guo J, Wang Q, Li Y, Chen Y, Yin X, Yang P, Lan F *, He QY *, Zhang G *, Wang T *. Finding Missing Proteins from the Epigenetically Manipulated Human Cell with Stringent Quality Criteria. Journal of Proteome Research (2015) 14(9):3645-5731Chen Y, Li Y, Zhong J, Zhang J, Chen Z, Yang L, Cao X, He QY *, Zhang G *, Wang T *. Identification of missing proteins defined by chromosome-centric proteome project in the cytoplasmic detergent-insoluble proteins. Journal of Proteome Research (2015) 14(9):3693-70932Zhong J, Xiao C, Gu W, Du G, Sun X, He QY *, Zhang G *. Transfer RNAs Mediate the Rapid Adaptation of Escherichia coli to Oxidative Stress. PLoS Genetics (2015) 11(6):e100530233Guo J, Lian X, Zhong J, Wang T *, Zhang G *. Length-dependent translation initiation benefits the functional proteome of human cells. Molecular BioSystems (2015) 11, 370-37834Chen Z, Yang L, Cui Y, Zhou Y, Yin X, Guo J, Zhang G *, Wang T *, He QY *. Cytoskeleton-centric protein transportation by exosomes transforms tumor-favorable macrophages. Oncotarget (2016) doi: 10.18632/oncotarget.11794.35Guo J, Cui Y, Yan Z, Luo Y, Zhang W, Deng S, Tang S, Zhang G, He QY *, Wang T *. Phosphoproteome Characterization of Human Colorectal Cancer SW620 Cell-Derived Exosomes and New Phosphosite Discovery for C-HPP. Journal of Proteome Research (2016) doi: 10.1021/acs.jproteome.6b0039136Lian X, Guo J, Gu W, Cui Y, Zhong J, Jin J, He QY *, Wang T *, Zhang G *. Genome-wide and experimental resolution of relative translation elongation speed at individual gene level in human cells. PLoS Genetics (2016) 12(2): e1005901.37Bartholomäus A, Fedyunin I, Feist P, Sin C, Zhang G, Valleriani A, Ignatova Z *. Bacteria differently regulate mRNA abundance to specifically respond to various stresses. Phil. Trans. R. Soc. A (2016) 374:20150069.39Yang XY, He K, Du G, Wu X, Yu G, Pan Y, Zhang G *, Sun X *, He QY *. Integrated Translatomics with Proteomics to Identify Novel Iron–Transporting Proteins in Streptococcus pneumoniae. Front. Microbiol. (2016) 7:78.40Zhao P, Zhong J, Liu W, Zhao J, Zhang G *. Protein-Level Integration Strategy of Multiengine MS Spectra Search Results for Higher Confidence and Sequence Coverage. Journal of Proteome Research (2017) 16(12), 4446–4454.41曹欣，张弓 * 超高精度大规模测序mapping算法FANSe在非模式生物中的应用 【综述】 中国科学·生命科学 (2017), 47. doi: 10.1360/N052016-00280 Cao X, Zhang G *. Application of the hyper-accurate mapping algorithm FANSe for next-generation sequencing in non-model organisms Sci Sin Vitae, 2017, 47, doi: 10.1360/N052016-0028042曹欣，刘婉婷，张弓 * 非模式细菌功能基因组获取策略比较 基因组学与应用生物学 (2017), 36(7): 1-8.43Wang C, Chen K, Liao S, Gu W, Lian X, Zhang J, Gao X, Liu X, Wang T, He QY *, Zhang G *, Liu L *. The flightless I protein interacts with RNA-binding proteins and is involved in the genome-wide mRNA post-transcriptional regulation in lung carcinoma cells. International Journal of Oncology (2017) doi: 10.3892/ijo.2017.3995.44Mai Z, Xiao C, Jin J, Zhang G *. Low-cost, Low-bias and Low-input RNA-seq with High Experimental Verifiability based on Semiconductor Sequencing. Scientific Reports (2017) 7:1053.45赵晶，张弓 * 翻译组学：方法及应用 【综述】 生命的化学 (2017), 37(1): 70-79. doi: 10.13488/j.smhx.2017011146Jia HL, Liu CW, Zhang L, Xu WJ, Gao XJ, Bai J, Xu YF, Xu M *, Zhang G *. Sets of serum exosomal microRNAs as candidate diagnostic biomarkers for Kawasaki disease. Scientific Reports (2017) 7:44706.47Zhang M, Zhao K, Xu X, Yang Y, Yan S, Wei P, Liu H, Xu J, Xiao F, Yang X, Huang N, Zhou H, Liu J, He K, Xie K, Zhang G, Huang S, Zhang N *. A Peptide Encoded by Circular Form of LINC-PINT Suppresses Oncogenic Transcriptional Elongation in Glioblastoma Nature Communications (2018) 9, 4475.48Li D, Lu S, Liu W, Zhao X, Mai Z, Zhang G *. Optimal settings of mass spectrometry open search strategy for higher confidence Journal of Proteome Research (2018) 17(11), 3719-3729.49Wu Q, Tian Y, et al. In vivo CRISPR screening unveils histone demethylase UTX as an important epigenetic regulator in lung tumorigenesis. PNAS (2018) 115 (17), E3978-E3986.50Cao K, Wang H, Li N, Cao X, He J, Zhang B, He QY *, Zhang G *, Sun X *. Two zinc-binding domains in the transporter AdcA from Streptococcus pyogenes facilitate high-affinity binding and fast transport of zinc. Journal of Biological Chemistry (2018) 293 (16), 6075-6089.51Bao X, Guo X, et al. Capturing the Interactome of Newly Transcribed RNA. Nature Methods (2018) 15, 213-220.52Huang W, Liu W, Jin J, Xiao Q, Lu R, Chen W, Xiong S *, Zhang G *. Steady-state structural fluctuation is a predictor of the necessity of pausing-mediated co-translational folding for small proteins. Biochem Biophys Res Commun (2018) 498(1), 186-192.53Liu W, Xiang L, Zheng T, Jin J, Zhang G *. TranslatomeDB: a comprehensive database and cloud-based analysis platform for translatome sequencing data. Nucleic Acids Research (2018) 46(D1), D206-212.54Lu S, Zhang J, Lian XL, Sun L, Meng K, Chen Y, Sun Z, Yin X, Li Y, Zhao J, Wang T *, Zhang G *, He QY * A Hidden Human Proteome Encoded by “Non-Coding” Genes Nucleic Acids Research (2019) 47 (15), 8111-8125.55Mai Z, Liu W, Ding W, Zhang G * Misassembly of long reads undermines de novo assembled ethnicity-specific genomes: Validation in a Chinese Han Population Human Genetics (2019) https://doi.org/10.1007/s00439-019-02032-6.56Zhao J, Zhang H, Qin B, Nikolay R, He QY, Spahn CMT *, Zhang G * Multifaceted stoichiometry control of bacterial operons revealed by data-independent acquisition mass spectrometry Frontiers in Genetics (2019) 10:473.57Shi T, Wei Q, Wang Z, Zhang G *, Sun X *, He QY * Photocatalytic protein damage by silver nanoparticles circumvents bacterial stress response and multidrug resistance mSphere (2019) 4(3), e00175-19.58Sun Y, Cai M, Zhong J, Jin F, Xiao J, Yang L, Wang Y, Zhang Q, Wang Z *, Zhang G *, Wang X * A long noncoding RNA lnc-ob1 facilitates bone formation by upregulating Osterix in osteoblasts Nature Metabolism (2019) 1, 485–496 (2019).59Gao L, Hu Y, et al. Lung Cancer Deficient in the Tumor Suppressor GATA4 are Sensitive To TGFBR1 Inhibition Nature Communications (2019) 10:1665.60Liao X, Zhao J, Liang S, Jin J, Li C, Xiao R, Li L, Guo M, Zhang G *, Lin Y *. Enhancing co-translational folding of heterologous protein by deleting non-essential ribosomal proteins in Pichia pastoris Biotechnology and Biofuels (2019) 12:38.61Zhao J, Qin B, Nikolay R, Spahn CMT, Zhang G *. Translatomics: The Global View of Translation International Journal of Molecular Sciences (2019) 20(1), 212.62Zhou Z #, Yang Z #, Ou J #, Zhang H, Zhang Q *, Dong M *, Zhang G * Temperature Dependence of the SARS-CoV-2 affinity to human ACE2 determines COVID-19 progression and clinical outcome Computational and Structural Biotechnology Journal (2020) 19:161-167.63Lin H #, Zhang G #, Zhang XC #, Lian X, Su J, Chen SL, Wu YL * Germline Variation Networks in the PI3K/AKT Pathway Corresponding to Familial High-incidence Lung Cancer Pedigrees BMC Cancer (2020) 9;20(1):1209.64Li D *, Liu B, Zheng H, Xiao X, Li Z, Luan E, Li W, Wang Y, Yang Y, Long Q, Song J, Zhang G * XY-Meta: A high-efficiency search engine for large scale metabolome annotation with accurate FDR estimation Analytical Chemistry (2020) 92:8, 5701-5707.65Lu S, Wang T, Zhang G, He QY * Understanding the proteome encoded by “non-coding RNAs”: New insights into human genome Science China Life Sciences (2020) 1-10.66Zhang G *, Zhang Y, Jin J * The Ultrafast and Accurate Mapping Algorithm FANSe3: Mapping a Human Whole-Genome Sequencing Dataset Within 30 min Phenomics (2021) 1, 22–30 .67Ou J, Zhou Z, Dai R, Zhang J, Zhao S, Wu X, Lan W, Ren Y, Cui L, Lan Q, Lu L, Seto D, Chodosh J, Wu J, Zhang G *, Zhang Q * V367F Mutation in SARS-CoV-2 Spike RBD Emerging during the Early Transmission Phase Enhances Viral Infectivity through Increased Human ACE2 Receptor Binding Affinity Journal of Virology (2021) 95(16):e0061721.68Man Zhang, Jing Zhao, Wanying Li, Shuqi Wen, Huiling Huang, Jie Dong, Bing Liu, Gong Zhang, Hong-Bin Wang, Yanting Shen *, Hong-Lei Jin * Increased photosystem II translation efficiency as an important photoprotective mechanism in an Arabidopsis thaliana ecotype (Tibet-0) adapted to high light environments Environmental and Experimental Botany (2021) 183:104350.69Yixi Li, Dehua Li, Yang Chen, Yongping Lu, Fangbin Zhou, Chunhong Li, Zhipeng Zeng, Wanxia Cai, Liewen Lin, Qiang Li, Mingjun Ye, Jingjing Dong, Lianghong Yin *, Donge Tang *, Gong Zhang *, Yong Dai * Robust Glycogene-Based Prognostic Signature for Proficient Mismatch Repair Colorectal Adenocarcinoma Frontiers in Oncology (2021) 11:727752.70Huiying Fang, Guandi Zeng, Jing Zhao, Tingkai Zheng, Lina Xu, Wei Gu, Yutong Liu, Jinning Zhang, Xuesong Sun *, Gong Zhang * Genome recombination-mediated tRNA up-regulation conducts general antibiotic resistance of bacteria at early stage Frontiers in Microbiology in press.71方慧颖，张弓 * 及与翻译调控的细菌耐药研究进展 微生物学通报 (2022) in press.^[3]

1、表达量高和活性强的CVN突变体的编码序列及其应用，国家发明专利（已授权），201310164451.4，发明人：陈伟、张弓、熊盛、金静洁，2014。

2、通过翻译暂停序列理性重设计改善蛋白质可溶性表达的方法，国家发明专利（已授权），201310164454.8，发明人：张弓、陈伟、熊盛、金静洁，2015。

3、完整冷冻保存及解冻细胞内核糖体新生肽链复合物的方法，国家发明专利（已授权），201410387213.4，发明人：孙黎、张弓、王通、金静洁，2016。

4、完整获取组织内核糖体新生肽链复合物的方法，国家发明专利（已授权），201410452522.5，发明人：孙黎、张弓、王通、金静洁，2017。

5、完整获取植物组织中核糖体新生肽链复合物的方法与应用，国家发明专利（已授权），201510332732.5，发明人：赵晶、张弓，2018。^[3]

国家级青年人才，省级青年人才。^[1]

广东省党外知识分子联谊会理事，深圳市龙华区第一届政协常委、高层次人才促进会会长、龙华区总商会常务理事。^[1]